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Isolation and characterisation of salivary microbiota of street dogs


Abstract:
Diseases spreading from animals to humans have become a major issue as a result of the easy means of transmission through water, air, food and direct contact. It was found that, there are a lot of risks associated with dog when humans come in contact with it. Many diseases are likely to spread from a common domestic animal, such as dog. In the present study, the saliva samples of street dogs from Thiruvottiyur area, Chennai were collected. Colonies were isolated by spread plate technique and further subjected to morphological and biochemical characterisation to identify the bacteria. In order to evaluate its pathogenicity against humans it was tested against 7 commercial antibiotics by disc diffusion method. Out of ten isolates, one strain named as S2 had shown resistance to all antibiotics. All the isolates from the saliva of dogs were predicted to be human pathogens and the strain S2 was further subjected to 16S rRNA sequencing and identified as Pasteurella multocida. It appears like street dogs might be the mediators to spread the pathogenic microbes easily to the humans.

Keywords:
Dog's saliva; Antibiotics; Pathogens; Microbes; Isolation

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How to cite this article:
Sivakami B, Ambarin Farizah B, Aishwarya T, Vaishnavi, Viveka Devi R, Sakthivel K. Isolation and characterisation of salivary microbiota of street dogs. Journal of Biological and Information Sciences. 2015. 4 (1). 1-5.
Available from: http://www.biolim.com/journals/BOJ001/001/BOJ001A0029.